RRB Laboratory Assistant Grade II Syllabus & CBT Exam Pattern 2026 | CEN 05/2026

RRB Laboratory Assistant Grade II Syllabus & CBT Exam Pattern 2026

Candidates preparing for the Laboratory Assistant Grade II post under RRB Paramedical CEN 05/2026 should prepare the prescribed Professional Ability syllabus along with the common CBT sections.

Annexure C of CEN 05/2026 lists 8 Professional Ability syllabus areas for Laboratory Assistant Grade II.

This article provides the official Professional Ability syllabus, CBT exam pattern and a practical preparation framework.

1. RRB Laboratory Assistant Grade II CBT Exam Pattern

The Single Stage Computer Based Test (CBT) is objective in nature.

The section-wise distribution given in CEN 05/2026 is indicative, and the official notification states that the question paper may vary.

Professional Ability

Questions : 70

Marks : 70

General Awareness

Questions : 10

Marks : 10

General Arithmetic + General Intelligence & Reasoning

Questions : 10

Marks : 10

General Science

Questions : 10

Marks : 10

Total Questions : 100

Total Marks : 100

Exam Duration : 90 minutes

Duration for scribe-eligible PwBD/PWD candidates : 120 minutes

Negative Marking : 1/3 of the marks allotted for each wrong answer

2. RRB Laboratory Assistant Grade II Professional Ability Syllabus 2026

Annexure C of CEN 05/2026 lists the following Professional Ability syllabus areas for Laboratory Assistant Grade II:

• Anatomy

• Physiology

• Biochemistry

• Fundamentals of Medical Laboratory Technology

• Microbiology

• Clinical biochemistry

• Pathology & Histopathology

• Haematology and Blood and bank

3. Important Note About the Official Syllabus

RRB provides the Professional Ability syllabus as subject headings.

CEN 05/2026 does not provide a chapter-by-chapter expansion or separate official topic-wise marks distribution for the Laboratory Assistant Grade II Professional Ability syllabus.

Candidates should therefore prepare all 8 prescribed syllabus areas rather than assuming that a particular area will carry a fixed number of questions.

The wording “Haematology and Blood and bank” is retained here because that is how the official Annexure C text is represented in the source material.

4. Suggested Preparation Order

The following grouping is an Edumozo preparation framework and should not be interpreted as official RRB weightage.

Every official syllabus area remains important and should be covered.

a. Foundation Sciences

• Anatomy

• Physiology

• Biochemistry

b. Laboratory Core

• Fundamentals of Medical Laboratory Technology

• Microbiology

• Clinical biochemistry

c. Diagnostic Laboratory Areas

• Pathology & Histopathology

• Haematology and Blood and bank

Candidates should also keep the common CBT sections active throughout their preparation:

• General Awareness

• General Arithmetic

• General Intelligence & Reasoning

• General Science

5. Four-Week Laboratory Assistant Grade II Study Plan

The following four-week structure is Edumozo study guidance.

Candidates can adjust the daily workload according to their available preparation time and existing subject strength.

Week 1

Primary Focus :

• Anatomy

• Physiology

• Biochemistry

Revision / Practice :

• Recall important concepts

• Continue common-section preparation

Week 2

Primary Focus :

• Fundamentals of Medical Laboratory Technology

• Microbiology

Revision / Practice :

• Objective-question practice

• Revision of Week 1 areas

Week 3

Primary Focus :

• Clinical biochemistry

• Pathology & Histopathology

Revision / Practice :

• Revision of previously completed areas

• Mixed objective-question practice

Week 4

Primary Focus :

• Haematology and blood-bank-related syllabus

Revision / Practice :

• Full Professional Ability revision

• Timed objective-question practice

Weekly Rule :

Reserve time every week for recall, objective-question practice and the common CBT sections.

Do not wait until the final week to begin revision.

6. Laboratory Assistant Grade II Revision Strategy

A simple three-stage approach can be used while preparing the Professional Ability syllabus.

Step 1 : Learn

Complete each official syllabus heading and prepare concise notes using trusted study sources.

Step 2 : Recall

Close the notes and try to retrieve important concepts from memory.

Revisit areas that you cannot explain or remember clearly.

Step 3 : Practice

Attempt objective questions under timed conditions.

When reviewing mistakes, identify whether the error occurred because of:

• A concept gap

• Confusion between answer options

• Calculation error

• Rushed reading

7. Do Not Ignore the Common CBT Sections

Professional Ability is the largest indicated section of the examination, with 70 questions and 70 marks.

However, candidates should continue preparing the common sections throughout their Laboratory Assistant Grade II preparation.

The common sections are:

• General Awareness

• General Arithmetic

• General Intelligence & Reasoning

• General Science

Under the indicative CBT distribution, these common areas together account for 30 questions.

They should therefore be practised regularly rather than being left entirely for the final stage of preparation.

8. Negative Marking and Accuracy

Incorrect answers attract a deduction of 1/3 of the marks allotted for each wrong answer.

Candidates should therefore develop both speed and accuracy during objective-question practice.

While practising, keep track of repeated mistakes and revise the concepts responsible for those errors.

Uncertain guesses should not be treated as free attempts because incorrect answers attract negative marking.

9. Laboratory Assistant Grade II Preparation Checklist

Before completing your preparation, check whether you have:

• Covered all 8 official Professional Ability syllabus areas

• Revised your weakest Professional Ability areas

• Practised Professional Ability objective questions

• Practised General Awareness

• Practised General Arithmetic

• Practised General Intelligence & Reasoning

• Practised General Science

• Identified repeated mistakes from objective-question practice

• Practised while accounting for 1/3 negative marking

• Completed a final rapid revision of your notes

10. Quick Summary

Recruitment : RRB Paramedical CEN 05/2026

Post : Laboratory Assistant Grade II

Professional Ability Syllabus Areas : 8

Exam Mode : Computer Based Test (CBT)

Total Questions : 100

Total Marks : 100

Professional Ability : 70 Questions / 70 Marks

General Awareness : 10 Questions / 10 Marks

General Arithmetic + General Intelligence & Reasoning : 10 Questions / 10 Marks

General Science : 10 Questions / 10 Marks

Duration : 90 Minutes

Negative Marking : 1/3 of the marks allotted for each wrong answer

11. Frequently Asked Questions

i. What is the RRB Laboratory Assistant Grade II syllabus for 2026?

The Professional Ability syllabus under CEN 05/2026 contains 8 official syllabus areas: Anatomy, Physiology, Biochemistry, Fundamentals of Medical Laboratory Technology, Microbiology, Clinical biochemistry, Pathology & Histopathology, and Haematology and Blood and bank.

ii. How many Professional Ability syllabus areas are there for RRB Laboratory Assistant Grade II?

Annexure C of CEN 05/2026 lists 8 Professional Ability syllabus areas for Laboratory Assistant Grade II.

iii. How many questions are asked from Professional Ability in the RRB Laboratory Assistant Grade II CBT?

The indicative CBT pattern assigns 70 questions carrying 70 marks to Professional Ability.

The official notification states that the section-wise distribution is indicative and the actual question paper may vary.

iv. What are the common subjects in the RRB Laboratory Assistant Grade II exam?

In addition to Professional Ability, the CBT includes General Awareness, General Arithmetic, General Intelligence & Reasoning and General Science.

v. Does RRB provide topic-wise marks or weightage for the Laboratory Assistant Grade II syllabus?

CEN 05/2026 provides the Professional Ability syllabus headings but does not prescribe a separate official topic-wise marks distribution for the 8 Laboratory Assistant Grade II syllabus areas.

Candidates should therefore prepare all the prescribed syllabus areas.

12. Official Source

The Laboratory Assistant Grade II Professional Ability syllabus and CBT information in this article are based on Railway Recruitment Boards CEN 05/2026, Recruitment of Paramedical Categories, including Annexure C.

The suggested preparation order, four-week study structure and revision strategy are Edumozo preparation guidance and are not official RRB topic-wise weightage.

Candidates should refer to CEN 05/2026 and subsequent official RRB notices as the authority for examination rules, amendments and updates.

Official RRB Website :

rrb.indianrailways.gov.in